IHC
Immunoperoxidase staining of formalin fixed, paraffin-embedded liver tissue of normal mouse fed with low fat diet (A) or choline-deficient high fat diet (B) showing F4/80 staining in Kupffer cells| Product Name | F4/80 Rat Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | he epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL. These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system. The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin. F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation. The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Rat |
| Tested Applications | ICC/IFIHCWB |
| ICC/IF:1:100~1:200 IHC:1:20~1:100 WB:1:250~1:1000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1ug/ul |
| Purification | Affinity purified |
| Gene Symbol | ADGRE1 |
|---|---|
| Gene Synonyms | Adhesion G protein-coupled receptor E1 |
| Target | Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. |
| Alternative Names | Adgre1 Adhesion G protein-coupled receptor E1 AGRE1_HUMAN EGF-like module receptor 1 EGF-like module-containing mucin-like hormone receptor-like 1 EMR1 hormone receptor |
| Molecular Weight(MW) | 160KD |
IHC
Immunoperoxidase staining of formalin fixed, paraffin-embedded liver tissue of normal mouse fed with low fat diet (A) or choline-deficient high fat diet (B) showing F4/80 staining in Kupffer cells| Application Notes | ICC/IF:1:100~1:200 IHC:1:20~1:100 WB:1:250~1:1000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
