WB
Western blot analysis using GPX4 antibody against Cos7(1) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using GPX4 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human liver tissues using GPX4 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using GPX4 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using GPX4 antibody with DAB staining.WB
Western blot analysis using GPX4 antibody against Mouse brain(1),Mouse heart(2),Rat stomach(3) tissue.| Product Name | Anti-GPX4 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GPX4 |
|---|---|
| Gene Synonyms | MCSP SMDS GPx-4 PHGPx snGPx GSHPx-4 snPHGPx |
| Gene Full Name | glutathione peroxidase 4 |
| Gene Summary | The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization. [provided by RefSeq, Dec 2018] |
| Molecular Weight(MW) | 22kDa |
| Cellular Localization | Cytoplasm, Mitochondrion |
| SwissProt ID | P36969 |
|---|
WB
Western blot analysis using GPX4 antibody against Cos7(1) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using GPX4 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human liver tissues using GPX4 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using GPX4 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using GPX4 antibody with DAB staining.
WB
Western blot analysis using GPX4 antibody against Mouse brain(1),Mouse heart(2),Rat stomach(3) tissue.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300. |
