IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using SHH antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human Kidney tissues using SHH antibody with DAB staining.WB
Western blot analysis using SHH antibody against NIH3T3(1) cell lysate.| Product Name | Anti-SHH antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SHH |
|---|---|
| Gene Synonyms | TPT HHG1 HLP3 HPE3 SMMCI ShhNC TPTPS MCOPCB5 |
| Gene Full Name | sonic hedgehog signaling molecule |
| Gene Summary | This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 50kDa |
| Cellular Localization | Cell membrane, Membrane |
| SwissProt ID | Q15465 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using SHH antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human Kidney tissues using SHH antibody with DAB staining.
WB
Western blot analysis using SHH antibody against NIH3T3(1) cell lysate.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300. |
