WB
Western blot analysis of LSM14A using anti-LSM14A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LSM14A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for LSM14A at approximately 60 kDa. The expected band size for LSM14A is at 51 kDa.ICC/IF
IF analysis of LSM14A using anti-LSM14A antibody. LSM14A was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-LSM14A Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).IP
Immunoprecipitating LSM14A in A549 whole cell lysate. Western blot analysis of LSM14A using anti- LSM14A antibody. Lane 1: A549 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- LSM14A antibody in A549 whole cell lysate, Lane 3: anti- LSM14A antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- LSM14A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Heavy Chain). The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for LSM14A at approximately 60 kDa. The expected band size for LSM14A is at 51 kDa.FC
Flow Cytometry analysis of SH-SY5Y cells using anti-LSM14A antibody. Overlay histogram showing SH-SY5Y cells stained with A06546-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LSM14A Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | LSM14A Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human LSM14A recombinant protein (Position: F105-R404). |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIPWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 IP:1:250-1:300 FC:1:50-200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LSM14A |
|---|---|
| Gene Synonyms | RAP55 FAM61A RAP55A C19orf13 |
| Gene Full Name | LSM14A mRNA processing body assembly factor |
| Gene Summary | Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM, Mar 2008] |
| Molecular Weight(MW) | 60kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of LSM14A using anti-LSM14A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LSM14A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for LSM14A at approximately 60 kDa. The expected band size for LSM14A is at 51 kDa.
ICC/IF
IF analysis of LSM14A using anti-LSM14A antibody. LSM14A was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-LSM14A Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).
IP
Immunoprecipitating LSM14A in A549 whole cell lysate. Western blot analysis of LSM14A using anti- LSM14A antibody. Lane 1: A549 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- LSM14A antibody in A549 whole cell lysate, Lane 3: anti- LSM14A antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- LSM14A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Heavy Chain). The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for LSM14A at approximately 60 kDa. The expected band size for LSM14A is at 51 kDa.
FC
Flow Cytometry analysis of SH-SY5Y cells using anti-LSM14A antibody. Overlay histogram showing SH-SY5Y cells stained with A06546-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LSM14A Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 IP:1:250-1:300 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
Data sheet for OM643872