WB
Dilution ratio of primary antibody: 1:1000, Incubation conditions of primary antibody: room temperature for 2 hours, Secondary antibody: HRP labeled Goat Anti Rabbit IgG (H+L) at 1:1000 Dilution. lysis buffer: (-) HeLa, (+) HeLa+Sodium Butyrate (30mM, 4hr). Protein loading amount: 20 μ g. Exposure time: 60 s .Theoretical molecular weight: Multiple. Actual molecular weight: Multiple.WB
Dilution ratio of primary antibody: 1:1000, Incubation conditions of primary antibody: 2 hours at room temperature. Secondary antibody: HRP labeled Goat Anti Rabbit IgG (H+L)at 1:1000 Dilution. lysis buffer: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Mouse liver, 5: Mouse kidney protein .loading amount: 20 μ g .exposure time: 60 s .theoretical molecular weight: Multiple .actual molecular weight: Multiple.IHC
Organization: Human colon cancer section type: Formaldehyde fixation¶ffin embedding repair method: High temperature and high pressure repair solution: Tris/EDTA (pH 9.0) primary antibody dilution: 1:500, primary antibody incubation conditions: room temperature for 1 hour, secondary antibody: Anti Rabbit and Mouse Polymer HRP (ready to use) Nuclear counterstaining: Hematoxylin (blue) Result description: Brown staining indicates a positive signal for Butyryl Lysine Rabbit Monoclonial Antibody.| Product Name | Butyryl-Lysine Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Protein A |
| Function | Lysine butyrylation structure is similar to lysine acetylation and acetylation, and is a recently discovered reversible modification that can regulate protein activity. Integrating proteomics and biochemical analysis techniques, a large number of literature reports have found that many proteins in prokaryotic and eukaryotic organisms, whether histone or non histone, undergo lysine acetylation modification. Many histone and non histone substrates such as p53 and p300/CBP undergo butyrylation modifications, suggesting that lysine butyrylation plays an important role in epigenetic regulation, such as chromatin dynamics and plasticity, DAN transcriptional regulation, and tumorigenesis. |
|---|
WB
Dilution ratio of primary antibody: 1:1000, Incubation conditions of primary antibody: room temperature for 2 hours, Secondary antibody: HRP labeled Goat Anti Rabbit IgG (H+L) at 1:1000 Dilution. lysis buffer: (-) HeLa, (+) HeLa+Sodium Butyrate (30mM, 4hr). Protein loading amount: 20 μ g. Exposure time: 60 s .Theoretical molecular weight: Multiple. Actual molecular weight: Multiple.
WB
Dilution ratio of primary antibody: 1:1000, Incubation conditions of primary antibody: 2 hours at room temperature. Secondary antibody: HRP labeled Goat Anti Rabbit IgG (H+L)at 1:1000 Dilution. lysis buffer: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Mouse liver, 5: Mouse kidney protein .loading amount: 20 μ g .exposure time: 60 s .theoretical molecular weight: Multiple .actual molecular weight: Multiple.
IHC
Organization: Human colon cancer section type: Formaldehyde fixation¶ffin embedding repair method: High temperature and high pressure repair solution: Tris/EDTA (pH 9.0) primary antibody dilution: 1:500, primary antibody incubation conditions: room temperature for 1 hour, secondary antibody: Anti Rabbit and Mouse Polymer HRP (ready to use) Nuclear counterstaining: Hematoxylin (blue) Result description: Brown staining indicates a positive signal for Butyryl Lysine Rabbit Monoclonial Antibody.| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 º C and avoid repeated freezing and thawing. |
Data sheet for OM644023