WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PKM antibody. The HRP conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549, Lane 2: NIH3T3, Lane 3: C6.
IHC
Mouse lung was stained with Anti-PKM rabbit antibody.
ICC/IF
Immunofluorescence analysis of rat-spleen tissue. 1,PKM2 Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.