Welcome to Omnimabs!
TEL: 02154156726 Info: info@Omnimabs.com CN
PKM Rabbit mAb
Catalog Number:OM644038
OM644038
Discount Price
OM644038-100ul
100ul
OM644038-50ul
50ul
OM644038-20ul
20ul
Product Profile
Product Name PKM Rabbit mAb
Antibody Type Primary Antibodies
Key Feature
Clonality monoclonal
Isotype IgG
Host Species Rabbit
Tested Applications ICC/IFIHCWB
WB:1:1000-1:5000
IHC:1:200-1:1000
ICC/IF:1:200-1:1000
Species Reactivity HumanMouseRat
Concentration 1mg/ml
Purification Protein A
Target Information
Gene Symbol PKM
Gene Synonyms PK3
TCB
p58
OIP3
PKM2
CTHBP
THBP1
HEL-S-30
Gene Full Name pyruvate kinase M1/2
Gene Summary This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
Molecular Weight(MW) 57kDa
Cellular Localization Cytoplasm,Nucleus.
Application

WB

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PKM antibody. The HRP conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549, Lane 2: NIH3T3, Lane 3: C6.

IHC

Mouse lung was stained with Anti-PKM rabbit antibody.

ICC/IF

Immunofluorescence analysis of rat-spleen tissue. 1,PKM2 Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
Application Notes WB:1:1000-1:5000
IHC:1:200-1:1000
ICC/IF:1:200-1:1000
Additional Information
Form Liquid
Storage Instructions -15°C to -25°C/1 year(Do not lower than -25°C)
Storage Buffer PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
validation
question title
question detaiil
Verification code
 

References(0)