WB
Dilution series of mCherry in HEK 293T cell lysate Primary antibody: 1:1,000 .Secondary antibody: goat anti-mouse Alexa Fluor® 647 1:1,000.| Product Name | Mouse anti RFP Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Red fluorescent protein (RFP) |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2c |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is a mouse (IgG2c) monoclonal antibody raised against RFP, reactive to a variety of RFP derivates, including mOrange, OFP, DsRed, mCherry, mRFP, mRFPRuby, mScarlet, mScarlet-I, tdTomato, and mPlum. |
|---|
WB
Dilution series of mCherry in HEK 293T cell lysate Primary antibody: 1:1,000 .Secondary antibody: goat anti-mouse Alexa Fluor® 647 1:1,000.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS, 50% glycerol, preservative: 0.09% sodium azide. |
Data sheet for OM644061