WB
Western blot of NIH 3T3 cells showing specific immunolabeling of the 53kDa Vimentin protein.ICC/IF
Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.| Product Name | Vim Chicken Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human vimentin purified from E. coli |
| Clonality | polyclonal |
|---|---|
| Isotype | IgY |
| Host Species | Chicken |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:500 |
|
| Species Reactivity | HumanMouse |
| Purification | Affinity purified |
| Gene Symbol | VIM |
|---|---|
| Gene Full Name | vimentin |
| Gene Summary | This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017] |
| Molecular Weight(MW) | 53kDa |
| Cellular Localization | Cytoplasm,Cytoplasm,cytoskeleton,Nucleus matrix,Cell membrane. |
WB
Western blot of NIH 3T3 cells showing specific immunolabeling of the 53kDa Vimentin protein.
ICC/IF
Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.| Application Notes | WB:1:1000 ICC/IF:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. Stable for 12 months from date of receipt. |
| Storage Buffer | Total IgY fraction in PBS + 10 mM NaN3. |
Data sheet for OM644167