WB
Western blot analysis of anti- Nesprin 2/SYNE2 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: monkey COS-7 whole cell lysates. Use rabbit anti- Nesprin 2/SYNE2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.IHC
IHC analysis of Nesprin 2/SYNE2 using anti-Nesprin 2/SYNE2 antibody. Nesprin 2/SYNE2 was detected in a paraffin-embedded section of human penis squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-Nesprin 2/SYNE2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
IF analysis of Nesprin 2/SYNE2 using anti-Nesprin 2/SYNE2 antibody. Nesprin 2/SYNE2 was detected in an immunocytochemical section of Hela cells. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)was used as secondary antibody. The section was counterstained with DAPI(Blue).FC
Flow Cytometry analysis of U87 cells using anti-Nesprin 2/SYNE2 antibody. Overlay histogram showing U87 cells stained with anti-Nesprin 2/SYNE2 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nesprin 2/SYNE2 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to Nesprin 2/SYNE2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Nesprin 2/SYNE2 recombinant protein (Position: Q3765-Q5590). |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYNE2 |
|---|---|
| Gene Synonyms | NUA EDMD5 KASH2 Nesp2 TROPH NUANCE SYNE-2 Nesprin-2 |
| Gene Full Name | spectrin repeat containing nuclear envelope protein 2 |
| Gene Summary | The protein encoded by this gene is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| Molecular Weight(MW) | 796kDa(Observed MW 50-60kDa) |
| Cellular Localization | Nucleus outer membrane,Sarcoplasmic reticulum membrane,Cell membrane,Cytoplasm, cytoskeleton,Mitochondrion,Nucleus, nucleoplasm,Cytoplasm, myofibril, sarcomere, Z line. |
WB
Western blot analysis of anti- Nesprin 2/SYNE2 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: monkey COS-7 whole cell lysates. Use rabbit anti- Nesprin 2/SYNE2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.
IHC
IHC analysis of Nesprin 2/SYNE2 using anti-Nesprin 2/SYNE2 antibody. Nesprin 2/SYNE2 was detected in a paraffin-embedded section of human penis squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-Nesprin 2/SYNE2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of Nesprin 2/SYNE2 using anti-Nesprin 2/SYNE2 antibody. Nesprin 2/SYNE2 was detected in an immunocytochemical section of Hela cells. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)was used as secondary antibody. The section was counterstained with DAPI(Blue).
FC
Flow Cytometry analysis of U87 cells using anti-Nesprin 2/SYNE2 antibody. Overlay histogram showing U87 cells stained with anti-Nesprin 2/SYNE2 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nesprin 2/SYNE2 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
Data sheet for OM644291