WB
Goat Anti-DNAJB9 (aa61-75) Antibody(1µg/ml) staining of Human Liver (A), Kidney (B) and -Mouse Liver (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.ICC/IF
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).FC
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.| Product Name | Goat Anti-DNAJB9 (aa61-75) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide with sequence C-PDAEAKFREIAEAYE, from the internal regoin of the protein sequence according to NP_036460.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | FCICC/IFWB |
| WB::1-3µg/ml ICC/IF:10µg/ml FC:10µg/ml |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DNAJB9 |
|---|---|
| Gene Synonyms | MDG1 ERdj4 MDG-1 MST049 MSTP049 |
| Gene Full Name | DnaJ heat shock protein family (Hsp40) member B9 |
| Gene Summary | This gene is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. This gene is a member of the type 2 subgroup of DnaJ proteins. The encoded protein is localized to the endoplasmic reticulum. This protein is induced by endoplasmic reticulum stress and plays a role in protecting stressed cells from apoptosis. [provided by RefSeq, Dec 2010] |
| Molecular Weight(MW) | 28kDa |
WB
Goat Anti-DNAJB9 (aa61-75) Antibody(1µg/ml) staining of Human Liver (A), Kidney (B) and -Mouse Liver (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
ICC/IF
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
FC
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.| Application Notes | WB::1-3µg/ml ICC/IF:10µg/ml FC:10µg/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Storage Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
