IF-F
Immunofluorescence analysis of frozen mouse brain tissue with Rat anti-Myelin PLP antibody at 1/1,000 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (488) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).IF-F
Immunofluorescence analysis of frozen mouse brain tissue with Rat anti-Myelin PLP antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (488) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rat anti-Myelin PLP antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Myelin PLP antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-Myelin PLP antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.WB
Western blot analysis of Myelin PLP on different lysates with Rat anti-Myelin PLP antibody at 1/5,000 dilution. Notice: no heat means the lysate is not boiled. Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.| Product Name | Myelin PLP Recombinant Antibody - Rat IgG1 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Myelin PLP aa 1-233. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Tested Applications | IF-FIHCWB |
| IF-F:1:1000 IHC:1:1000 WB:1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PLP1 |
|---|---|
| Gene Synonyms | PLP PMD HLD1 MMPL SPG2 GPM6C PLP/DM20 |
| Gene Full Name | proteolipid protein 1 |
| Gene Summary | This gene encodes a transmembrane proteolipid protein that is the predominant component of myelin. The encoded protein may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations in this gene cause Pelizaeus-Merzbacher disease and spastic paraplegia type 2. Alternatively splicing results in multiple transcript variants, including the DM20 splice variant. [provided by RefSeq, Feb 2015] |
| Alternative Names | HLD1 antibody Lipophilin antibody Major myelin proteolipid protein antibody MMPL antibody Myelin proteolipid protein antibody MYPR_HUMAN antibody PLP 1 antibody PLP antibody PLP/DM20 antibody PLP1 antibody HLD1 antibody Lipophilin antibody Major myelin proteolipid protein antibody MMPL antibody Myelin proteolipid protein antibody MYPR_HUMAN antibody PLP 1 antibody PLP antibody PLP/DM20 antibody PLP1 antibody PMD antibody Proteolipid protein 1 antibody SPG2 antibody |
| Molecular Weight(MW) | 30 kDa(Observed band size: 25/20 kDa) |
| Cellular Localization | Cell membrane, Myelin membrane. |
IF-F
Immunofluorescence analysis of frozen mouse brain tissue with Rat anti-Myelin PLP antibody at 1/1,000 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (488) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).
IF-F
Immunofluorescence analysis of frozen mouse brain tissue with Rat anti-Myelin PLP antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (488) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).
IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rat anti-Myelin PLP antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Myelin PLP antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-Myelin PLP antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
WB
Western blot analysis of Myelin PLP on different lysates with Rat anti-Myelin PLP antibody at 1/5,000 dilution. Notice: no heat means the lysate is not boiled. Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.| Application Notes | IF-F:1:1000 IHC:1:1000 WB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
