IF-F
Application: IHC-Fr, Species: Rat, Site: pancreas, Sample: Frozen section, Antibody concentration: 1/200, Antigen retrieval: Not required.IF-P
Application: IF-Tissue, Species: Human, Site: pancreas, Sample: Paraffin-embedded section, Antibody concentration: 1/500.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-PGP9.5 antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-PGP9.5 antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.WB
Western blot analysis of PGP9.5 on different lysates with Mouse anti-PGP9.5 antibody at 1/10,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of SH-SY5Y cells labeling PGP9.5 with Mouse anti-PGP9.5 antibody at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PGP9.5 antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (594) were used as the secondary antibody at 1/1,000 dilution.| Product Name | PGP9.5 Recombinant Antibody - Mouse IgG1 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human PGP95 aa 191-223 / 223. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Tested Applications | ICC/IFIF-FIF-PIHCWB |
| IF-F:1:200 IF-P:1:500 IHC:1:5000 WB:1:10000 ICC/IF:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | UCHL1 |
|---|---|
| Gene Synonyms | NDGOA PARK5 PGP95 SPG79 PGP9.5 SPG79A UCHL-1 Uch-L1 HEL-117 PGP 9.5 HEL-S-53 |
| Gene Full Name | ubiquitin C-terminal hydrolase L1 |
| Gene Summary | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009] |
| Alternative Names | Epididymis luminal protein 117 antibody Epididymis secretory protein Li 53 antibody HEL 117 antibody HEL S 53 antibody NDGOA antibody Neuron cytoplasmic protein 9.5 antibody OTTHUMP00000218137 antibody OTTHUMP00000218139 antibody OTTHUMP00000218140 antibody OTTHUMP00000218141 antibody Park 5 antibody PARK5 antibody PGP 9.5 antibody PGP9.5 antibody PGP95 antibody Protein gene product 9.5 antibody Ubiquitin C terminal esterase L1 antibody Ubiquitin C terminal hydrolase antibody Ubiquitin C terminal hydrolase L1 antibody Ubiquitin carboxyl terminal esterase L1 antibody Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody Ubiquitin thioesterase L1 antibody Ubiquitin thiolesterase antibody Ubiquitin thiolesterase L1 antibody UCH-L1 antibody UCHL1 antibody UCHL1_HUMAN antibody |
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum membrane. |
IF-F
Application: IHC-Fr, Species: Rat, Site: pancreas, Sample: Frozen section, Antibody concentration: 1/200, Antigen retrieval: Not required.
IF-P
Application: IF-Tissue, Species: Human, Site: pancreas, Sample: Paraffin-embedded section, Antibody concentration: 1/500.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-PGP9.5 antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-PGP9.5 antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
WB
Western blot analysis of PGP9.5 on different lysates with Mouse anti-PGP9.5 antibody at 1/10,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of SH-SY5Y cells labeling PGP9.5 with Mouse anti-PGP9.5 antibody at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PGP9.5 antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (594) were used as the secondary antibody at 1/1,000 dilution.| Application Notes | IF-F:1:200 IF-P:1:500 IHC:1:5000 WB:1:10000 ICC/IF:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
