WB
Western blot analysis of Parvalbumin on different lysates with Chicken anti-Parvalbumin antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Parvalbumin Recombinant Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Rabbit anti-Chicken IgY-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.WB
Western blot analysis of Parvalbumin on rat skeletal muscle tissue lysate with Chicken anti-Parvalbumin antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Parvalbumin Recombinant Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Rabbit anti-Chicken IgY-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Parvalbumin Recombinant Antibody, 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IF-F
Application: Immunofluorescence (IHC-Fr). Species: Mouse. Tissue: Cerebellum. Sample: Frozen section. Antigen retrieval: Not required. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Parvalbumin Recombinant Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Chicken IgY (488), 1.5 hours at room temperature.IF-F
Application: Immunofluorescence (IHC-Fr). Species: Rat. Tissue: Cerebellum. Sample: Frozen section. Antigen retrieval: Not required. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Parvalbumin Recombinant Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Chicken IgY (488), 1.5 hours at room temperature.FC
Application: Flow Cytometry (Intra). Species: Human. Sample: SK-MEL-28 (Human malignant melanoma cell)/RPMI8226 (Human multiple myeloma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: Parvalbumin Recombinant Antibody(1/1,000, Red), 15 minutes at room temperature. Secondary antibody: Goat Anti-Chicken IgY (488), 15 minutes at room temperature. Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.| Product Name | Parvalbumin Recombinant Antibody - Chicken IgY (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Paravalbumin aa 1-110 / 110. |
| Isotype | IgY |
|---|---|
| Tested Applications | FCIF-FIHCWB |
| WB:1:5000 IHC:1:2000 IF-F:1:500 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PVALB |
|---|---|
| Gene Synonyms | D22S749 |
| Gene Full Name | parvalbumin |
| Gene Summary | The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] |
| Alternative Names | D22S749 antibody MGC116759 antibody Parvalbumin alpha antibody PRVA_HUMAN antibody PVALB antibody |
| Molecular Weight(MW) | 12 kDa |
| Cellular Localization | Axon, cytoplasm, synapse. |
WB
Western blot analysis of Parvalbumin on different lysates with Chicken anti-Parvalbumin antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Parvalbumin Recombinant Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Rabbit anti-Chicken IgY-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
WB
Western blot analysis of Parvalbumin on rat skeletal muscle tissue lysate with Chicken anti-Parvalbumin antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Parvalbumin Recombinant Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Rabbit anti-Chicken IgY-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Parvalbumin Recombinant Antibody, 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IF-F
Application: Immunofluorescence (IHC-Fr). Species: Mouse. Tissue: Cerebellum. Sample: Frozen section. Antigen retrieval: Not required. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Parvalbumin Recombinant Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Chicken IgY (488), 1.5 hours at room temperature.
IF-F
Application: Immunofluorescence (IHC-Fr). Species: Rat. Tissue: Cerebellum. Sample: Frozen section. Antigen retrieval: Not required. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Parvalbumin Recombinant Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Chicken IgY (488), 1.5 hours at room temperature.
FC
Application: Flow Cytometry (Intra). Species: Human. Sample: SK-MEL-28 (Human malignant melanoma cell)/RPMI8226 (Human multiple myeloma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: Parvalbumin Recombinant Antibody(1/1,000, Red), 15 minutes at room temperature. Secondary antibody: Goat Anti-Chicken IgY (488), 15 minutes at room temperature. Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.| Application Notes | WB:1:5000 IHC:1:2000 IF-F:1:500 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
