IHC
IHC image of SIGLEC15 Recombinant Monoclonal Antibody diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.ICC/IF
Immunofluorescence staining of PC-3 cell with SIGLEC15 Recombinant Monoclonal Antibody at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.| Product Name | SIGLEC15 Recombinant Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human SIGLEC15 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Tested Applications | ICC/IFIHC |
| IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIGLEC15 |
|---|---|
| Gene Synonyms | CD33L3 HsT1361 SIGLEC-15 |
| Gene Full Name | sialic acid binding Ig like lectin 15 |
| Gene Summary | Predicted to be involved in regulation of actin cytoskeleton organization; regulation of bone resorption; and regulation of osteoclast development. Predicted to be located in membrane. Predicted to be part of protein-containing complex. Predicted to be active in plasma membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | CD33 antigen-like 3 antibody CD33 molecule-like 3 antibody CD33L3 antibody HsT1361 antibody Sialic acid-binding Ig-like lectin 15 antibody SIG15_HUMAN antibody Siglec-15 antibody SIGLEC15 antibody |
| Cellular Localization | Membrane. |
IHC
IHC image of SIGLEC15 Recombinant Monoclonal Antibody diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
ICC/IF
Immunofluorescence staining of PC-3 cell with SIGLEC15 Recombinant Monoclonal Antibody at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.| Application Notes | IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
