IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Olig2 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-Olig2 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Application: IHC-Fr. Species: Mouse. Site: brain. Sample: Frozen section. Antibody concentration: 1/200. Antigen retrieval: Not required.| Product Name | Olig2 Recombinant Antibody - Rat IgG1 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Olig2 aa 238-287 / 323. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Tested Applications | IF-FIHC |
| IHC:1:500 IF-F:1:200 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | OLIG2 |
|---|---|
| Gene Synonyms | BHLHB1 OLIGO2 RACK17 PRKCBP2 bHLHe19 |
| Gene Full Name | oligodendrocyte transcription factor 2 |
| Gene Summary | This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. [provided by RefSeq, Jul 2008] |
| Alternative Names | Basic domain helix loop helix protein class B 1 antibody Basic helix loop helix protein class B 1 antibody BHLHB antibody bHLHB1 antibody bHLHe19 antibody Class B basic helix loop helix protein 1 antibody Class B basic helix-loop-helix protein 1 antibody class E basic helix loop helix protein 19 antibody Class E basic helix-loop-helix protein 19 antibody Human protein kinase C binding protein RACK17 antibody Olig2 antibody OLIG2_HUMAN antibody Oligo2 antibody Oligodendrocyte lineage transcription factor 2 antibody Oligodendrocyte specific bHLH transcription factor 2 antibody Oligodendrocyte transcription factor 2 antibody OTTHUMP00000067569 antibody OTTHUMP00000067570 antibody PRKCBP2 antibody Protein kinase C binding protein 2 antibody Protein kinase C binding protein RACK17 antibody Protein kinase C-binding protein 2 antibody Protein kinase C-binding protein RACK17 antibody RACK17 antibody |
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
| SwissProt ID | Q9EQW6 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Olig2 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-Olig2 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Application: IHC-Fr. Species: Mouse. Site: brain. Sample: Frozen section. Antibody concentration: 1/200. Antigen retrieval: Not required.| Application Notes | IHC:1:500 IF-F:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
