WB
Western blot analysis using ROCK1 antibody against Cos7(1), A431(2), K562(3) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using ROCK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using ROCK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using ROCK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using ROCK1 antibody with DAB staining.| Product Name | Anti-ROCK1 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ROCK1 |
|---|---|
| Gene Synonyms | ROCK-I P160ROCK |
| Gene Full Name | Rho associated coiled-coil containing protein kinase 1 |
| Gene Summary | This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 158kDa |
| Cellular Localization | Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane |
| SwissProt ID | Q13464 |
|---|
WB
Western blot analysis using ROCK1 antibody against Cos7(1), A431(2), K562(3) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using ROCK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using ROCK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using ROCK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using ROCK1 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
