WB
Western blot analysis using PGK1 antibody against Hela(1), Hepg2(2), K562(3) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using PGK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using PGK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using PGK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using PGK1 antibody with DAB staining.WB
Western blot analysis using PGK1 antibody against Hela(1),Hepg2(2),K562(3),Cos-7(4),Nih3t3(5),Mcf-7(6),T24(7),U251(8),B16(9) cell lysate.| Product Name | Anti-PGK1 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PGK1 |
|---|---|
| Gene Synonyms | PGKA MIG10 HEL-S-68p |
| Gene Full Name | phosphoglycerate kinase 1 |
| Gene Summary | The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. |
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P00558 |
|---|
WB
Western blot analysis using PGK1 antibody against Hela(1), Hepg2(2), K562(3) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using PGK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using PGK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using PGK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using PGK1 antibody with DAB staining.
WB
Western blot analysis using PGK1 antibody against Hela(1),Hepg2(2),K562(3),Cos-7(4),Nih3t3(5),Mcf-7(6),T24(7),U251(8),B16(9) cell lysate.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
