IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using XBP1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissuess using XBP1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissuess using XBP1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissuess using XBP1 antibody with DAB staining.| Product Name | Anti-XBP1 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | XBP1 |
|---|---|
| Gene Synonyms | XBP2 TREB5 XBP-1 TREB-5 |
| Gene Full Name | X-box binding protein 1 |
| Gene Summary | This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. |
| Molecular Weight(MW) | 29 kDa |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus |
| SwissProt ID | P17861 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using XBP1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissuess using XBP1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissuess using XBP1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissuess using XBP1 antibody with DAB staining.| Application Notes | IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
