ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using SPI1 antibody against Hepg2(1),Hek293(2) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | SPI1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SPI1 |
|---|---|
| Gene Synonyms | AGM10 Dis-1 Dis1 OF PU.1 Sfpi-1 SFPI1 SPI-1 SPI-A Tcfpu1 Tfpu.1 |
| Gene Full Name | Spi-1 proto-oncogene |
| Gene Summary | This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | 31 kDa transforming protein 31 kDa-transforming protein 31kDa Transforming Protein hCG_25181 hematopoietic transcription factor PU.1 PU.1 protein SFFV proviral integration 1 SFFV proviral integration 1 protein SPIA spleen focus forming virus (SFFV) proviral integration oncogene spleen focus forming virus (SFFV) proviral integration oncogene spi1 spleen focus forming virus proviral integration oncogene spi1 Transcription Factor spi1 |
| Molecular Weight(MW) | 42kDa |
| Source | Rabbit |
| Cellular Localization | Nucleus |
| SwissProt ID | P17947 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using SPI1 antibody against Hepg2(1),Hek293(2) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
