ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using HSP90 antibody against Hela(1),Hepg2(2) cell,Mouse skin tissue(3),Mouse kidney tissue(4),Mouse brain tissue(5),Mouse liver tissue(6),Mouse lung tissue(7),Mouse spleen tissue(8) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | HSP90 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HSP90AA1 |
|---|---|
| Gene Synonyms | 86kDa 89kDa EL52 HEL-S-65p Hsp103 hsp4 HSP86 Hsp86-1 Hsp89 HSP89A Hsp90 HSP90A HSP90N HSPC1 HSPCA HSPCAL1 HSPCAL4 HSPN LAP-2 LAP2 |
| Gene Full Name | heat shock protein 90 alpha family class A member 1 |
| Gene Summary | The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| Alternative Names | epididymis luminal secretory protein 52 epididymis secretory sperm binding protein Li 65p heat shock 86 kDa heat shock 90kD protein 1 alpha heat shock 90kD protein 1 alpha-like 4 heat shock 90kD protein alpha-like 4 heat shock 90kDa protein 1 alpha Heat shock protein heat shock protein 1 alpha heat shock protein 86 heat shock protein 90 alpha (cytosolic) class A member 1 heat shock protein 90kDa alpha (cytosolic) class A member 1 heat shock protein 90kDa alpha family class A member 1 heat shock protein family C member 1 heat shock protein HSP90 alpha heat shock protein 1 heat shock protein 86 kDa 1 heat shock protein 89 kDa Heat shock protein90 heat-shock protein hsp86 HSP HSP 86 HSP 90 alpha HSP86 lipopolysaccharide-associated protein 2 LPS-associated protein 2 renal carcinoma antigen NY-REN-38 TSTA tumor-specific transplantation 86 kDa antigen |
| Molecular Weight(MW) | 85kDa |
| Source | Rabbit |
| Cellular Localization | Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus |
| SwissProt ID | P07900 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using HSP90 antibody against Hela(1),Hepg2(2) cell,Mouse skin tissue(3),Mouse kidney tissue(4),Mouse brain tissue(5),Mouse liver tissue(6),Mouse lung tissue(7),Mouse spleen tissue(8) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
