ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using ANP32A antibody against K562(1),A549(2),A431(3),Huvec(4),Nih3t3(5) cell,Rat liver tissue(6),Rat lung tissue(7), Rat stomach tissue(8),Rat heart tissue(9) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | ANP32A Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ANP32A |
|---|---|
| Gene Synonyms | Anp32 C15orf1 HPPCn I1PP2A LANP MAPM PHAP1 PHAPI PP32 |
| Gene Full Name | acidic nuclear phosphoprotein 32 family member A |
| Gene Summary | Enables RNA binding activity. Involved in nucleocytoplasmic transport. Located in endoplasmic reticulum; nucleus; and perinuclear region of cytoplasm. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Acid nuclear phosphoprotein 32 (leucine rich) acidic (leucine-rich) nuclear phosphoprotein 32 family member A acidic nuclear phosphoprotein 32 acidic nuclear phosphoprotein pp32 cerebellar leucine rich acidic nuclear protein epididymis secretory sperm binding protein hepatopoietin Cn I1PP-2A inhibitor-1 of protein phosphatase-2A leucine-rich acidic nuclear protein leucine-rich acidic nuclear protein LANP mapmodulin MGC119787 MGC150373 potent heat-stable protein phosphatase 2A inhibitor I1PP2A putative HLA-DR-associated protein I putative human HLA class II associated protein I vacidic (leucine-rich) nuclear phosphoprotein 32 family member A |
| Molecular Weight(MW) | 29kDa |
| Source | Rabbit |
| Cellular Localization | Cytoplasm,Nucleus |
| SwissProt ID | P39687 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using ANP32A antibody against K562(1),A549(2),A431(3),Huvec(4),Nih3t3(5) cell,Rat liver tissue(6),Rat lung tissue(7), Rat stomach tissue(8),Rat heart tissue(9) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
