ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using USP9x antibody against Hela(1) ,K562(2),Hek293(3),Jurkat(4)cell . 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | USP9X Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | USP9X |
|---|---|
| Gene Synonyms | 5730589N07Rik DFFRX FAF FAF-X Fafl FAM hFAM MRX99 MRXS99F RGD1560056 XLID99 |
| Gene Full Name | ubiquitin specific peptidase 9 X-linked |
| Gene Summary | This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Alternative Names | deubiquitinating enzyme FAF-X Drosophila fat facets related X-linked fat facets homolog fat facets in mammals fat facets protein related X-linked fat facets protein-related X-linked fat facets-like X-linked fats facets protein related X probable ubiquitin carboxyl-terminal hydrolase FAF-X similar to Homo sapiens ubiquitin hydrolase encoded by GenBank Accession Number X98296 ubiquitin carboxyl-terminal hydrolase FAM ubiquitin specific peptidase 9 X chromosome ubiquitin specific protease 9 X chromosome (fat facets-like Drosophila) ubiquitin thioesterase FAF-X ubiquitin thiolesterase FAF-X ubiquitin-specific processing protease FAF-X ubiquitin-specific protease 9 X chromosome ubiquitin-specific-processing protease FAF-X unknown |
| Molecular Weight(MW) | 292kDa |
| Source | Rabbit |
| Cellular Localization | Cell projection, Cytoplasm, Cytoskeleton |
| SwissProt ID | Q93008 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using USP9x antibody against Hela(1) ,K562(2),Hek293(3),Jurkat(4)cell . 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
