ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using GDF11 + GDF8/Myostatin antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6),Nih3t3(7),Mcf-7(8),Raji(9) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | GDF11 + GDF8/Myostatin Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GDF11/MSTN |
|---|---|
| Gene Synonyms | GDF11 BMP11 Bone morphogenetic protein 11 GDF-11 BMP-11 Myostatin GDF8 MSTN |
| Gene Full Name | growth differentiation factor 11/myostatin |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein plays a role in the development of the nervous and other organ systems, and may regulate aging. [provided by RefSeq, Aug 2016]/This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jul 2016] |
| Alternative Names | BMP 11 antibody BMP-11 antibody BMP11 antibody Bone morphogenetic protein 11 antibody GDF 11 antibody GDF-11 antibody Gdf11 antibody GDF11_HUMAN antibody Growth differentiation factor 11 antibody Growth/differentiation factor 11 antibody |
| Molecular Weight(MW) | 45kDa |
| Source | Rabbit |
| Cellular Localization | Secreted |
| SwissProt ID | O95390，O14793 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using GDF11 + GDF8/Myostatin antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6),Nih3t3(7),Mcf-7(8),Raji(9) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
