ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using Mimitin antibody against Hela(1),Hepg2(2),K562(3),A549(4),Huvec(5),Nih3t3(6),Jurkat(7)cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Mimitin Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NDUFAF2 |
|---|---|
| Gene Synonyms | 1810058I14Rik B17.2L MC1DN10 mimitin MMTN NDUFA12L RGD1560158 |
| Gene Full Name | NADH:ubiquinone oxidoreductase complex assembly factor 2 |
| Gene Summary | NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene cause progressive encephalopathy resulting from mitochondrial complex I deficiency. [provided by RefSeq, Jul 2008] |
| Alternative Names | B172-like B172L mimitin mitochondrial MMTN Myc-induced mitochondria protein Myc-induced mitochondrial protein NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 2 isoform NDUFAF2_1 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 2 isoform NDUFAF2_3 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 2 isoform NDUFAF2_4 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 2 NADH dehydrogenase (ubiquinone) complex I assembly factor 2 NADH dehydrogenase 1 alpha subcomplex assembly factor 2 NDUFA12-like protein possibly related to cleft palate |
| Molecular Weight(MW) | 20kDa |
| Source | Rabbit |
| Cellular Localization | Mitochondrion |
| SwissProt ID | Q8N183 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using Mimitin antibody against Hela(1),Hepg2(2),K562(3),A549(4),Huvec(5),Nih3t3(6),Jurkat(7)cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
