ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using TFAP2A antibody against Hela(1),A549(2),A431(3),Huvec(4),Nih3t3(5)cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | TFAP2A Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TFAP2A |
|---|---|
| Gene Synonyms | AP-2 Ap-2 (a) AP-2alpha Ap2 AP2alpha AP2TF BOFS Tcfap2a TFAP2 |
| Gene Full Name | transcription factor AP-2 alpha |
| Gene Summary | The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009] |
| Alternative Names | activating enhancer-binding protein 2 alpha activating enhancer-binding protein 2-alpha activator protein 2 AP-2 transcription factor DNA-binding protein (AP-2) ERF-1 FLJ51761 OTTHUMP00000214240 OTTHUMP00000214243 transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha) |
| Molecular Weight(MW) | 48kDa |
| Source | Rabbit |
| Cellular Localization | Nucleus |
| SwissProt ID | P05549 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using TFAP2A antibody against Hela(1),A549(2),A431(3),Huvec(4),Nih3t3(5)cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
