ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using NR2F2 antibody against K562(1),A549(2),A431(3),Huvec(4),Nih3t3(5),Cos-7(6),C2C12(7)cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | NR2F2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NR2F2 |
|---|---|
| Gene Synonyms | 2700033K02Rik 9430015G03Rik Aporp1 ARP-1 ARP1 CHTD4 COUP-TF2 COUP-TFII COUPTF2 COUPTFB COUPTFII EAR3 NF-E3 SRXX5 SVP40 Tcfcoup2 TFCOUP2 |
| Gene Full Name | nuclear receptor subfamily 2 group F member 2 |
| Gene Summary | This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| Alternative Names | ADP-ribosylation factor related protein 1 apolipoprotein A-I regulatory protein 1 apolipoprotein AI regulatory protein 1 apolipoprotein regulatory protein 1 ARP-1 chicken ovalbumin chicken ovalbumin upstream promoter transcription factor 2 chicken ovalbumin upstream promoter-transcription factor I COUP transcription factor II COUP-TF II COUP-TF2 COUPb MGC117452 ovalbumin upstream promoter beta nuclear receptor |
| Molecular Weight(MW) | 46kDa |
| Source | Rabbit |
| Cellular Localization | Nucleus |
| SwissProt ID | P24468 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using NR2F2 antibody against K562(1),A549(2),A431(3),Huvec(4),Nih3t3(5),Cos-7(6),C2C12(7)cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
