ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using Actin antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6),Pc-12(7),Jurkat(8) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Actin Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ACTA1 |
|---|---|
| Gene Synonyms | ACTA Acta-2 Acts Actsk-1 ASMA CFTD CFTD1 CFTDM CMYO2A CMYO2B CMYO2C CMYP2A CMYP2B CMYP2C MPFD NEM1 NEM2 NEM3 SHPM |
| Gene Full Name | actin alpha 1, skeletal muscle |
| Gene Summary | The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause a variety of myopathies, including nemaline myopathy, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects with manifestations such as hypotonia. [provided by RefSeq, Sep 2019] |
| Alternative Names | a-actin ACTA1 actin actin alpha 1 alpha-actin alpha-actin-1 nemaline myopathy type 3 skeletal muscle alpha-actin |
| Molecular Weight(MW) | 42kDa |
| Source | Rabbit |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P68133 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using Actin antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6),Pc-12(7),Jurkat(8) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
