WB
Western blot analysis using PGCP antibody against U251(1),A549(2),Nih3t3(3) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | PGCP Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CPQ |
|---|---|
| Gene Synonyms | 1190003P12Rik 2610034C17Rik Hls2 LAL Lal-1 LDP PGCP |
| Gene Full Name | carboxypeptidase Q |
| Gene Summary | This gene encodes a metallopeptidase that belongs to the peptidase M28 family. The encoded protein may catalyze the cleavage of dipeptides with unsubstituted terminals into amino acids. [provided by RefSeq, Jul 2013] |
| Alternative Names | aminopeptidase blood plasma glutamate carboxypeptidase hematopoietic lineage switch 2 hematopoietic lineage switch 2 related protein hls2-rp LAL liver annexin like-1 liver annexin-like protein 1 lysosomal dipeptidase plasma glutamate carboxypeptidase secretory protein Ser-Met dipeptidase |
| Molecular Weight(MW) | 51kDa（observed：60kDa) |
| Cellular Localization | Endoplasmic reticulum;Golgi apparatus;Lysosome;Secreted |
| SwissProt ID | Q9Y646 |
|---|
WB
Western blot analysis using PGCP antibody against U251(1),A549(2),Nih3t3(3) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300. |
