ICC/IF
Immunofluorescence analysis of Hela cells using GCKantibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Product Name | Anti-GCK antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | AF488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFFC |
| IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GCK |
|---|---|
| Gene Synonyms | GK GLK HK4 HHF3 HKIV HXKP LGLK MODY2 PNDM1 FGQTL3 |
| Gene Full Name | glucokinase |
| Gene Summary | This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia. [provided by RefSeq, Aug 2017] |
| Molecular Weight(MW) | 52kDa |
| Cellular Localization | Cytoplasm, Mitochondrion, Nucleus |
| SwissProt ID | P35557 |
|---|
ICC/IF
Immunofluorescence analysis of Hela cells using GCKantibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Application Notes | IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
