WB
Western blot analysis using RAP1GAP antibody against Mcf-7(1),Hek293(2),B16(3),P19(4),A549(5) cell lysate and Mouse liver(6) lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human brain tissues using RAP1GAP antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using RAP1GAP antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using RAP1GAP antibody with DAB staining.| Product Name | Anti-RAP1GAP antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RAP1GAP |
|---|---|
| Gene Synonyms | RAPGAP RAP1GA1 RAP1GAP1 RAP1GAPII |
| Gene Full Name | RAP1 GTPase activating protein |
| Gene Summary | This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Aug 2011] |
| Molecular Weight(MW) | 73kDa |
| Cellular Localization | Golgi apparatus, Membrane |
| SwissProt ID | P47736 |
|---|
WB
Western blot analysis using RAP1GAP antibody against Mcf-7(1),Hek293(2),B16(3),P19(4),A549(5) cell lysate and Mouse liver(6) lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human brain tissues using RAP1GAP antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using RAP1GAP antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using RAP1GAP antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
