WB
Western blot analysis using ERK1/2 antibody against Hepg2(1),C2C12(2),Cos-7(3),Hek293(4),B16(5),P19(6),A549(7) cell lysate and Mouse intestine(8),Mouse brain(9) lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using ERK1/2 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using ERK1/2 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using ERK1/2 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using ERK1/2 antibody with DAB staining.| Product Name | Anti-ERK1/2 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAPK1 |
|---|---|
| Gene Synonyms | ERK p38 p40 p41 ERK2 ERT1 NS13 ERK-2 MAPK2 PRKM1 PRKM2 P42MAPK p41mapk p42-MAPK |
| Gene Full Name | mitogen-activated protein kinase 1 |
| Gene Summary | This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014] |
| Molecular Weight(MW) | 42kDa,44kDa |
| Cellular Localization | Nucleus,Cytoplasm,Membrane |
| SwissProt ID | P28482 |
|---|
WB
Western blot analysis using ERK1/2 antibody against Hepg2(1),C2C12(2),Cos-7(3),Hek293(4),B16(5),P19(6),A549(7) cell lysate and Mouse intestine(8),Mouse brain(9) lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using ERK1/2 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using ERK1/2 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using ERK1/2 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using ERK1/2 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
