WB
Western blot analysis using alpha Actinin 4 antibody against Hela(1),Hepg2(2),Mcf-7(3),A549(4),C2C12(5),U251(6) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using alpha Actinin 4 antibody with DAB staining.WB
Western blot analysis using alpha Actinin 4 antibody against Mouse lung (1),Mouse kidney(2),Mouse heart(3),Mouse brain(4),Mouse intestine(5)lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using alpha Actinin 4 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using alpha Actinin 4 antibody with DAB staining.| Product Name | Anti-alpha Actinin 4 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ACTN4 |
|---|---|
| Gene Synonyms | Alpha-actinin-4 Non-muscle alpha-actinin 4 ACTN4 |
| Gene Full Name | actinin alpha 4 |
| Gene Summary | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 105kDa |
| Cellular Localization | Cell junction,Cytoplasm,Nucleus |
| SwissProt ID | O43707 |
|---|
WB
Western blot analysis using alpha Actinin 4 antibody against Hela(1),Hepg2(2),Mcf-7(3),A549(4),C2C12(5),U251(6) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using alpha Actinin 4 antibody with DAB staining.
WB
Western blot analysis using alpha Actinin 4 antibody against Mouse lung (1),Mouse kidney(2),Mouse heart(3),Mouse brain(4),Mouse intestine(5)lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using alpha Actinin 4 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using alpha Actinin 4 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
