IHC
Immunohistochemical analysis of paraffin-embedded Human human cervical cancer tissues using mtTFA antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human human Kidney tissues using mtTFA antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human human lung cancer tissues using mtTFA antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human human renal carcinoma tissues using mtTFA antibody with DAB staining.WB
Western blot analysis using mtTFA antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),Cos-7(5),P19(6),B16(7),C2C12(8),K562(9),Jurkat(10),Mfc(11),Nih3t3(12),T24(13),Raji(14) cell. gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with| Product Name | Anti-mtTFA antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TFAM |
|---|---|
| Gene Synonyms | TCF6 MTTF1 MTTFA TCF6L1 TCF6L2 TCF6L3 MTDPS15 |
| Gene Full Name | transcription factor A, mitochondrial |
| Gene Summary | This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Molecular Weight(MW) | 29kDa |
| Source | Rabbit |
| Cellular Localization | Mitochondrion |
| SwissProt ID | Q00059 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human human cervical cancer tissues using mtTFA antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human human Kidney tissues using mtTFA antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human human lung cancer tissues using mtTFA antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human human renal carcinoma tissues using mtTFA antibody with DAB staining.
WB
Western blot analysis using mtTFA antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),Cos-7(5),P19(6),B16(7),C2C12(8),K562(9),Jurkat(10),Mfc(11),Nih3t3(12),T24(13),Raji(14) cell. gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
