IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using SPHK2 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using SPHK2 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal tissues using SPHK2 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using SPHK2 antibody with DAB staining.WB
Western blot analysis using SPHK2 antibody against A549(1),Cos-7(2),B16(3),K562(4),Jurkat(5),Mfc(6),Raji(7) cell.WB
Western blot analysis using SPHK2 antibody against Mouse testes(1),Mouse stomach(2),Rat stomach(3),Rat serum(4),Rat lung(15) tissue.| Product Name | Anti-SPHK2 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SPHK2 |
|---|---|
| Gene Synonyms | SPHK2 SK-2 SPK 2 SPK-2 SK2 |
| Gene Full Name | sphingosine kinase 2 |
| Gene Summary | This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] |
| Molecular Weight(MW) | 69kDa(observed:55kDa) |
| Source | Rabbit |
| Cellular Localization | Cytoplasm,Nucleus |
| SwissProt ID | Q9NRA0 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using SPHK2 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using SPHK2 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal tissues using SPHK2 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using SPHK2 antibody with DAB staining.
WB
Western blot analysis using SPHK2 antibody against A549(1),Cos-7(2),B16(3),K562(4),Jurkat(5),Mfc(6),Raji(7) cell.
WB
Western blot analysis using SPHK2 antibody against Mouse testes(1),Mouse stomach(2),Rat stomach(3),Rat serum(4),Rat lung(15) tissue.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
