WB
Western blot analysis using GSK3B mouse monoclonal antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),B16(5) cell lysate .IHC
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Phospho GSK3P antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded colon cancer tissues using Phospho GSK3P antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Phospho GSK3P antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Phospho GSK3P antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using Phospho GSK3P antibody with DAB staining.| Product Name | Anti-Phospho-GSK3β(Ser9) antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Conjugation | poly-HRP |
| Modification | p-Ser9 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GSK3B |
|---|---|
| Gene Full Name | glycogen synthase kinase 3 beta |
| Gene Summary | The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017] |
| Molecular Weight(MW) | 47kDa |
| Function | Glycogen synthase kinase-3α (GSK-3α) and GSK-3β are highly similar isoforms of serine/ threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3β activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser 21 and Ser 9, respectively, through a phosphatidylinositol 3-kinase (PI 3-K)-protein kinase B (PKB)-dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser 9 of GSK-3β and inactivates both forms. GSK-3α/β is positively regulated by phosphorylation on Tyr 279 and Tyr 216, respectively. Activated GSK-3α/β participates in energy metabolism, neuronal cell development, and body pattern formation. Tyrosine dephosphorylation of GSK-3 is involved in its extracellular signal-dependent inactivation. |
| Cellular Localization | Cell membrane,Cytoplasm,Membrane,Nucleus. |
WB
Western blot analysis using GSK3B mouse monoclonal antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),B16(5) cell lysate .
IHC
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Phospho GSK3P antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded colon cancer tissues using Phospho GSK3P antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Phospho GSK3P antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Phospho GSK3P antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using Phospho GSK3P antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
