IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using XBP-1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using XBP-1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human Kidney tissues using XBP-1 antibody with DAB staining.WB
Western blot analysis using XBP-1 antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6),Nih3t3(7),Cos-7(8),C2C12(9),Hek293(10),Mcf-7(11),Pc-12(12),Jurkat(13) cell .WB
Western blot analysis using XBP-1 antibody against Mouse kidney tissue(1),Mouse brain tissue(2),Mouse lung tissue(3),Mouse spleen tissue(4),Mouse stomach tissue(5),Rat brain tissue(6),Rat stomach tissue(7) .| Product Name | Anti-XBP-1 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | XBP1 |
|---|---|
| Gene Synonyms | XBP1 TREB5 XBP2 X-box-binding protein 1 XBP-1 Tax-responsive element-binding protein 5 |
| Gene Full Name | X-box binding protein 1 |
| Gene Summary | This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 29kDa(observed:40kDa) |
| Source | Rabbit |
| Cellular Localization | Nucleus,Cytoplasm |
| SwissProt ID | P17861 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using XBP-1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using XBP-1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human Kidney tissues using XBP-1 antibody with DAB staining.
WB
Western blot analysis using XBP-1 antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6),Nih3t3(7),Cos-7(8),C2C12(9),Hek293(10),Mcf-7(11),Pc-12(12),Jurkat(13) cell .
WB
Western blot analysis using XBP-1 antibody against Mouse kidney tissue(1),Mouse brain tissue(2),Mouse lung tissue(3),Mouse spleen tissue(4),Mouse stomach tissue(5),Rat brain tissue(6),Rat stomach tissue(7) .| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
