WB
Western blot analysis using GAPDH antibody against Hela(1),Cos-7(2),Mcf-7(3),B16(4),Nih3t3(5) cell.IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GAPDH antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using GAPDH antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using GAPDH antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using GAPDH antibody with DAB staining.| Product Name | Anti-GAPDH antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Conjugation | HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | GAPDH |
|---|---|
| Gene Synonyms | G3PD GAPD HEL-S-162eP |
| Gene Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Gene Summary | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
| Molecular Weight(MW) | 37kDa |
| Cellular Localization | Cytoplasm,Cytoskeleton,Membrane,Nucleus. |
| SwissProt ID | P04406 |
|---|
WB
Western blot analysis using GAPDH antibody against Hela(1),Cos-7(2),Mcf-7(3),B16(4),Nih3t3(5) cell.
IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GAPDH antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using GAPDH antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using GAPDH antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using GAPDH antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide.Purified antibody in PBS with 0.05% PC300. |
