IF-P
Immunohistochemical analysis of paraffin-embedded Human human prostate cancer tissues using HSP70 antibody with DAB staining. with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature . Incubate the tissue with the primary antibody diluted at a ratio of 1:150 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:150 and incubate for one hour. Tyramine labeled with 488 (Green,OM642679) was used as chromogenic agent, and DAPI(Blue,OM642679) was used for double dyeing, and the anti-fluorescence attenuation tablets were sealed.| Product Name | Anti-HSP70 antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Conjugation | AF488 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | IFFC |
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | HSPA1A |
|---|---|
| Gene Synonyms | HSP70 HSP72 HSPA1 HSP70I HSP70-1 HSP70-2 HSP70.1 HSP70.2 HSP70-1A HEL-S-103 |
| Gene Full Name | heat shock protein family A (Hsp70) member 1A |
| Gene Summary | This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 70kDa |
| Cellular Localization | Cytoplasm,Cytoskeleton,Nucleus,Secreted. |
| SwissProt ID | P0DMV8 |
|---|
IF-P
Immunohistochemical analysis of paraffin-embedded Human human prostate cancer tissues using HSP70 antibody with DAB staining. with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature . Incubate the tissue with the primary antibody diluted at a ratio of 1:150 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:150 and incubate for one hour. Tyramine labeled with 488 (Green,OM642679) was used as chromogenic agent, and DAPI(Blue,OM642679) was used for double dyeing, and the anti-fluorescence attenuation tablets were sealed.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide.Purified antibody in PBS with 0.05% PC300. |
