WB
Western blot analysis using GRP78/Bip antibody against Huvec(1)Thp-1(2),Mcf-7(3),Hela(4),Hepg2(5),A549(6),Hek293(7),Jurkat(8),Cos-7(9),K562(10),A431(11) lysate .IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GRP78/Bip antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Kidney tissues using GRP78/Bip antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GRP78/Bip antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using GRP78/Bip antibody with DAB staining.| Product Name | Anti-GRP78/Bip antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Conjugation | poly-HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | HSPA5 |
|---|---|
| Gene Synonyms | BIP GRP78 HEL-S-89n |
| Gene Full Name | heat shock protein family A (Hsp70) member 5 |
| Gene Summary | The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers. [provided by RefSeq, Jul 2020] |
| Molecular Weight(MW) | 73kDa |
| Cellular Localization | Endoplasmic reticulum lumen,Melanosome,Cytoplasm,Cell surface. |
| SwissProt ID | P11021 |
|---|
WB
Western blot analysis using GRP78/Bip antibody against Huvec(1)Thp-1(2),Mcf-7(3),Hela(4),Hepg2(5),A549(6),Hek293(7),Jurkat(8),Cos-7(9),K562(10),A431(11) lysate .
IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GRP78/Bip antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Kidney tissues using GRP78/Bip antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GRP78/Bip antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using GRP78/Bip antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide.Purified antibody in PBS with 0.05% PC300. |
