WB
Western blot analysis using IDE/Insulin Degrading Enzyme antibody against PC-12(1)lysate .IHC
Immunohistochemical analysis of paraffin-embedded Human Kidney tissues using IDE/Insulin Degrading Enzyme antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human liver tissues using IDE/Insulin Degrading Enzyme antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using IDE/Insulin Degrading Enzyme antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using IDE/Insulin Degrading Enzyme antibody with DAB staining.| Product Name | Anti-IDE/Insulin Degrading Enzyme antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Conjugation | poly-HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | IDE |
|---|---|
| Gene Synonyms | INSULYSIN |
| Gene Full Name | insulin degrading enzyme |
| Gene Summary | This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009] |
| Molecular Weight(MW) | 118kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Membrane,Secreted. |
| SwissProt ID | P14735 |
|---|
WB
Western blot analysis using IDE/Insulin Degrading Enzyme antibody against PC-12(1)lysate .
IHC
Immunohistochemical analysis of paraffin-embedded Human Kidney tissues using IDE/Insulin Degrading Enzyme antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human liver tissues using IDE/Insulin Degrading Enzyme antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using IDE/Insulin Degrading Enzyme antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using IDE/Insulin Degrading Enzyme antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide.Purified antibody in PBS with 0.05% PC300. |
