IF-P
Immunohistochemical analysis of paraffin-embedded Human human breast cancer tissues using AMPKα1 antibody with DAB staining. with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature . Incubate the tissue with the primary antibody diluted at a ratio of 1:150 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:150 and incubate for one hour. Tyramine labeled with 488 (Green,OM642679) was used as chromogenic agent, and DAPI(Blue,OM642679) was used for double dyeing, and the anti-fluorescence attenuation tablets were sealed.| Product Name | Anti-AMPKα1 antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Conjugation | AF488 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | IFFC |
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | PRKAA1 |
|---|---|
| Gene Synonyms | AMPK AMPKa1 AMPK alpha 1 |
| Gene Full Name | protein kinase AMP-activated catalytic subunit alpha 1 |
| Gene Summary | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 62kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
| SwissProt ID | Q13131 |
|---|
IF-P
Immunohistochemical analysis of paraffin-embedded Human human breast cancer tissues using AMPKα1 antibody with DAB staining. with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature . Incubate the tissue with the primary antibody diluted at a ratio of 1:150 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:150 and incubate for one hour. Tyramine labeled with 488 (Green,OM642679) was used as chromogenic agent, and DAPI(Blue,OM642679) was used for double dyeing, and the anti-fluorescence attenuation tablets were sealed.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide.Purified antibody in PBS with 0.05% PC300. |
