WB
Western blot analysis using AMPKα1 antibody against Raji(1),C2C12(2),Nih3t3(3),Sk-ov-3(4),Hela(5),A431(6),Mouse spleen tissue(7),Rat spleen tissue(8),Mouse liver tissue(9),Rat liver tissue(10),Mouse adipose tissue(11),Mouse kidney tissue(12),Rat kidney tissue(13) lysate .IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using AMPKα1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using AMPKα1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human Kidney tissues using AMPKα1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using AMPKα1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using AMPKα1 antibody with DAB staining.| Product Name | Anti-AMPKα1 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Conjugation | HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | PRKAA1 |
|---|---|
| Gene Synonyms | AMPK AMPKa1 AMPK alpha 1 |
| Gene Full Name | protein kinase AMP-activated catalytic subunit alpha 1 |
| Gene Summary | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 62kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
| SwissProt ID | Q13131 |
|---|
WB
Western blot analysis using AMPKα1 antibody against Raji(1),C2C12(2),Nih3t3(3),Sk-ov-3(4),Hela(5),A431(6),Mouse spleen tissue(7),Rat spleen tissue(8),Mouse liver tissue(9),Rat liver tissue(10),Mouse adipose tissue(11),Mouse kidney tissue(12),Rat kidney tissue(13) lysate .
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using AMPKα1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using AMPKα1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human Kidney tissues using AMPKα1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using AMPKα1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using AMPKα1 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide.Purified antibody in PBS with 0.05% PC300. |
