WB
Western blot analysis using TBP antibody against Cos-7(1),Hek293(2),Pc-12(3) cell .WB
Western blot analysis using TBP antibody against Mouse kidney tissue(1),Mouse liver tissue(2) .IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using TBP antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using TBP antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using TBP antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using TBP antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using TBP antibody with DAB staining.| Product Name | Anti-TBP antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TBP |
|---|---|
| Gene Synonyms | HDL4 GTF2D SCA17 TFIID GTF2D1 |
| Gene Full Name | TATA-box binding protein |
| Gene Summary | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016] |
| Molecular Weight(MW) | 38kDa |
| Source | Rabbit |
| Cellular Localization | Nucleus |
| SwissProt ID | P20226 |
|---|
WB
Western blot analysis using TBP antibody against Cos-7(1),Hek293(2),Pc-12(3) cell .
WB
Western blot analysis using TBP antibody against Mouse kidney tissue(1),Mouse liver tissue(2) .
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using TBP antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using TBP antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using TBP antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using TBP antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using TBP antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
