WB
Western blot analysis using GAPDH antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6),Nih3t3(7),Cos-7(8),C2C12(9),Hek293(10),Mcf-7(11),Pc-12(12),Jurkat(13),Raji(14) cell .WB
Western blot analysis using GAPDH antibody against Mouse kidney tissue(1),Mouse brain tissue(2),Mouse liver tissue(3),Mouse lung tissue(4),Mouse heart tissue(5),Rat brain tissue(6),Rat lung tissue(7),Rat stomach tissue(8),Rat heart tissue(9).IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using GAPDH antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using GAPDH antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using GAPDH antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using GAPDH antibody with DAB staining.WB
Western blot analysis using GAPDH antibody against Hela(1),Cos-7(2),K562(3),B16(4),Hepg2(5),U251(6),A549(7) .WB
Western blot analysis using GAPDH antibody against Hela(1),Cos-7(2),K562(3),B16(4),Hepg2(5),U251(6),A549(7),Nih3t3(8),Mouse brain tissue(9),Mouse liver tissue(10),Mouse lung tissue(11) .WB
Western blot analysis using GAPDH antibody against Hela(1),Cos-7(2),K562(3),B16(4),Hepg2(5),A549(6),Rat testes tissue(7).WB
Western blot analysis using GAPDH antibody against K562(1),A549(2),Nih3t3(3),C2C12(4),Mcf-7(5),Pc-12(6),Mouse lung tissue(7),Mouse liver tissue(8),Rat spleen tissue(9) .WB
Western blot analysis using GAPDH antibody against MFC(1),P19(2),T24(3),Jurkat(4),Hepg2(5),ID8(6),PC-12(7),Mouse kidney tissue(8),Mouse liver tissue(9) ,Rat lung tissue(10),Rat stomach tissue(11).| Product Name | Anti-GAPDH antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GAPDH |
|---|---|
| Gene Synonyms | BARS-38 bb02e05 cb350 cb609 fb71f08 fk58c09 G3PD G3PDH GAPD Gapd-s GAPD2 gapdh GAPDH-2 GAPDS HEL-S-162eP HEL-S-278 HSD-35 I79_001391 KNC-NDS6 mg:bb02e05 wu:fb33a10 wu:fb71f08 wu:fk58c09 wu:ft80f05 zgc:76908 |
| Gene Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Gene Summary | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
| Alternative Names | 37 kd protein 38 kDa BFA-dependent ADP-ribosylation substrate AAG9 aging-associated gene 9 protein EC 1.2.1.12 epididymis secretory protein Li 278 epididymis secretory sperm binding protein Li 162eP GAPA GAPA-1 GAPC GAPC-1 GAPC1 GAPCP-1 GAPCp1 GAPD GAPDH GAPDH-2 GAPDH-A glceraldehyde-3-phosphate dehydrogenase glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12) glyceraldehyde 3-phosphate dehydrogenase testis-specific glyceraldehyde phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) glyceraldehyde-3-phosphate dehydrogenase type 2 glyceraldehyde-3-phosphate dehydrogenase spermatogenic glyceraldehyde-3-phosphate-dehydrogenase (EC 1.2.1.12) HGNC:4141 MGC88685 multifunctional protein glycolytic enzyme OCAS p38 component Oct1 coactivator in S phase 38 Kd component Peptidyl-cysteine S-nitrosylase GAPDH similar to Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA with GenBank Accession Number M33197.1 spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2 spermatogenic glyceraldehyde-3-phosphate dehydrogenase |
| Molecular Weight(MW) | 36kDa |
| Source | Rabbit |
| Cellular Localization | Cytoplasm, Cytoskeleton, Membrane, Nucleus |
| SwissProt ID | P04406 |
|---|
WB
Western blot analysis using GAPDH antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6),Nih3t3(7),Cos-7(8),C2C12(9),Hek293(10),Mcf-7(11),Pc-12(12),Jurkat(13),Raji(14) cell .
WB
Western blot analysis using GAPDH antibody against Mouse kidney tissue(1),Mouse brain tissue(2),Mouse liver tissue(3),Mouse lung tissue(4),Mouse heart tissue(5),Rat brain tissue(6),Rat lung tissue(7),Rat stomach tissue(8),Rat heart tissue(9).
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using GAPDH antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using GAPDH antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using GAPDH antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using GAPDH antibody with DAB staining.
WB
Western blot analysis using GAPDH antibody against Hela(1),Cos-7(2),K562(3),B16(4),Hepg2(5),U251(6),A549(7) .
WB
Western blot analysis using GAPDH antibody against Hela(1),Cos-7(2),K562(3),B16(4),Hepg2(5),U251(6),A549(7),Nih3t3(8),Mouse brain tissue(9),Mouse liver tissue(10),Mouse lung tissue(11) .
WB
Western blot analysis using GAPDH antibody against Hela(1),Cos-7(2),K562(3),B16(4),Hepg2(5),A549(6),Rat testes tissue(7).
WB
Western blot analysis using GAPDH antibody against K562(1),A549(2),Nih3t3(3),C2C12(4),Mcf-7(5),Pc-12(6),Mouse lung tissue(7),Mouse liver tissue(8),Rat spleen tissue(9) .
WB
Western blot analysis using GAPDH antibody against MFC(1),P19(2),T24(3),Jurkat(4),Hepg2(5),ID8(6),PC-12(7),Mouse kidney tissue(8),Mouse liver tissue(9) ,Rat lung tissue(10),Rat stomach tissue(11).| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
