ICC/IF
Immunofluorescence analysis of Hela cells using Alpha B Crystallin antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Product Name | Anti-Alpha B Crystallin antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | AF488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IDH2 |
|---|---|
| Gene Synonyms | AACRYA CMD1II Crya-2 CRYA2 CTPP2 CTRCT16 HEL-S-101 HSPB5 MFM2 MFM2A MFM2B P23 |
| Gene Full Name | crystallin alpha B |
| Gene Summary | Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2019] |
| Alternative Names | alpha crystallin B Alpha(B)-crystallin alpha-B-crystallin Alpha-crystallin B chain alpha-crystallin B chain-like protein Cryab CryAB antibody crystallin alpha 2 crystallin alpha B Crystallin alpha polypeptide 2 epididymis secretory protein Li 101 heat shock 20 kDa protein-like protein heat shock protein beta-5 heat shock protein beta-5 heat shock protein CryAB heat shock protein family B member 5 heat shock protein HspB5 heat-shock 20 kD like-protein HspB5 protein ORF1 ORF2 ORF32 renal carcinoma antigen NY-REN-27 Rosenthal fiber component similar to human alpha B-crystallin Rosenthal fiber component encoded by GenBank Accession Number S45630 |
| Molecular Weight(MW) | 20kDa |
| Source | Rabbit |
| Cellular Localization | Nucleus, Cytoplasm |
| SwissProt ID | P02511 |
|---|
ICC/IF
Immunofluorescence analysis of Hela cells using Alpha B Crystallin antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
