WB
Western blot analysis using PDHA1 antibody against Hela(1),K562(2),A549(3),A431(4),Huvec(5),Nih3t3(6),Cos-7(7),C2C12(8),Hek293(9),Mcf-7(10),Pc-12(11),Jurkat(12),Raji(13) cell .WB
Western blot analysis using PDHA1 antibody against Mouse skin tissue(1),Mouse kidney tissue(2),Mouse brain tissue(3),Mouse lung tissue(4),Mouse stomach tissue(5),Mouse heart tissue(6),Rat brain tissue(7),Rat liver tissue(8) .IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using PDHA1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using PDHA1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using PDHA1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissues using PDHA1 antibody with DAB staining.| Product Name | Anti-PDHA1 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PDHA1 |
|---|---|
| Gene Synonyms | E1alpha PDHA Pdha-1 PDHAD PDHCE1A PHE1A |
| Gene Full Name | pyruvate dehydrogenase E1 subunit alpha 1 |
| Gene Summary | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010] |
| Alternative Names | dehydrogenase PDHE1-A type I pyruvate dehydrogenase pyruvate dehydrogenase (lipoamide) alpha 1 pyruvate dehydrogenase alpha 1 pyruvate dehydrogenase alpha subunit precursor (EC 1.2.4.1) pyruvate dehydrogenase complex pyruvate dehydrogenase complex E1-alpha polypeptide 1 pyruvate dehydrogenase E1 alpha 1 pyruvate dehydrogenase E1 alpha 1 subunit pyruvate dehydrogenase E1 component alpha subunit pyruvate dehydrogenase E1-alpha precursor pyruvate dehydrogenase E1alpha subunit |
| Molecular Weight(MW) | 43kDa |
| Source | Rabbit |
| Cellular Localization | Mitochondrion matrix |
| SwissProt ID | P08559 |
|---|
WB
Western blot analysis using PDHA1 antibody against Hela(1),K562(2),A549(3),A431(4),Huvec(5),Nih3t3(6),Cos-7(7),C2C12(8),Hek293(9),Mcf-7(10),Pc-12(11),Jurkat(12),Raji(13) cell .
WB
Western blot analysis using PDHA1 antibody against Mouse skin tissue(1),Mouse kidney tissue(2),Mouse brain tissue(3),Mouse lung tissue(4),Mouse stomach tissue(5),Mouse heart tissue(6),Rat brain tissue(7),Rat liver tissue(8) .
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using PDHA1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using PDHA1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using PDHA1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissues using PDHA1 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
