ICC/IF
Immunofluorescence analysis of C2C12 cells using RPE65 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Product Name | Anti-RPE65 antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | AF488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RPE65 |
|---|---|
| Gene Synonyms | 65kDa A930029L06Rik BCO3 LCA2 Mord1 mRPE65 p63 rd12 RP20 sRPE65 |
| Gene Full Name | retinoid isomerohydrolase RPE65 |
| Gene Summary | The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital. [provided by RefSeq, Oct 2017] |
| Alternative Names | all-trans-retinyl-palmitate hydrolase BCO family member 3 lutein isomerase meso-zeaxanthin isomerase modifier of retinal degeneration 1 RBP-binding membrane protein retinal pigment epithelium 65 retinal pigment epithelium 65-like retinal pigment epithelium abundant protein RPE65 retinal pigment epithelium specific protein 65 retinal pigment epithelium 65 kDa retinal pigment epithelium-specific 65 kDa protein retinal pigment epithelium-specific 65kD protein retinal pigment epithelium-specific protein (65kD) retinal pigment epithelium-specific protein 65 retinal pigment epithelium-specific protein 65kDa Retinitis pigmentos retinitis pigmentosa 20 (autosomal recessive) retinol isomerase RPE65 rpe65 gene for retinal pigment epithelium-specific protein RPE65 retinoid isomerohydrolase truncated RPE65 |
| Molecular Weight(MW) | 61kDa |
| Source | Rabbit |
| Cellular Localization | Cytoplasm,Cell membrane |
| SwissProt ID | Q16518 |
|---|
ICC/IF
Immunofluorescence analysis of C2C12 cells using RPE65 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
