ICC/IF
Immunofluorescence analysis of Hela cells using Ikaros antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Product Name | Anti-Ikaros antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | AF488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IKZF1 |
|---|---|
| Gene Synonyms | 5832432G11Rik CVID13 hlk-1 Hs.54452 IK1 IKAROS LyF-1 LYF1 mKIAA4227 PPP1R92 PRO0758 Zfpn1a1 ZNFN1A1 |
| Gene Full Name | IKAROS family zinc finger 1 |
| Gene Summary | This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014] |
| Alternative Names | CLL-associated antigen KW-6 hIk-1 ikaros family zinc finger protein 1 Ikaros zinc finger protein zinc finger protein subfamily 1A 1 DNA-binding protein Ikaros CLL-associated antigen KW-6 Ikaros family zinc finger protein 1 lymphoid transcription factor LyF-1 lymphocyte-restricted zinc finger DNA binding protein lymphoid transcription factor LyF-1 predicted protein of HQ0758 protein phosphatase 1 regulatory subunit 92 transcription factor activity Ikaros Zfpn1a1 Ik11 zinc finger protein subfamily 1A 1 (Ikaros) zinc finger transcription factor |
| Molecular Weight(MW) | 58kDa |
| Source | Rabbit |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | Q13422 |
|---|
ICC/IF
Immunofluorescence analysis of Hela cells using Ikaros antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
