WB
Western blot analysis using RAD50 antibody against A549(1),A431(2),Nih3t3(3),C2C12(4),Hek293(5),Mcf-7(6),Raji(7)cell .IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using RAD50 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using RAD50 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using RAD50 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using RAD50 antibody with DAB staining.| Product Name | Anti-RAD50 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RAD50 |
|---|---|
| Gene Synonyms | hRad50 NBSLD RAD502 |
| Gene Full Name | RAD50 double strand break repair protein |
| Gene Summary | The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010] |
| Alternative Names | hsRAD50 RAD50 2 RAD50 homolog double strand break repair protein RAD50 PEN RAD50-2 RAD50-3 |
| Molecular Weight(MW) | 154kDa(observed：130kDa) |
| Cellular Localization | Nucleus |
| SwissProt ID | Q92878 |
|---|
WB
Western blot analysis using RAD50 antibody against A549(1),A431(2),Nih3t3(3),C2C12(4),Hek293(5),Mcf-7(6),Raji(7)cell .
IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using RAD50 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using RAD50 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using RAD50 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using RAD50 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
