WB
Western blot analysis using RF1 antibody against Rat spleen tissue(1) .IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using RF1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using RF1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using RF1 antibody with DAB staining.| Product Name | Anti-RF1 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Antigen peptide affinity chromatography |
| Gene Symbol | ETF1 |
|---|---|
| Gene Synonyms | D5S1995 D6Ertd109e ERF ERF1 RF1 SUP45L1 TB3-1 |
| Gene Full Name | eukaryotic translation termination factor 1 |
| Gene Summary | This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. [provided by RefSeq, Aug 2013] |
| Alternative Names | eRF1 eukaryotic release factor 1 polypeptide chain release factor 1 protein Cl1 sup45 (yeast omnipotent suppressor 45) homolog-like 1 |
| Molecular Weight(MW) | 49kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P62495 |
|---|
WB
Western blot analysis using RF1 antibody against Rat spleen tissue(1) .
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using RF1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using RF1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using RF1 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
